The Molding Melanin Magic mentorship program through TCU Pre-Health is geared to impact minority female student populations at the Texas Academy of Biomedical Sciences (TABS) in Fort Worth. The program provides small group mentorship as high school students are paired with a college student in their area of interest. Along with mentorship, workshops are utilized as a method of increasing confidence, exposure, and overall knowledge about college and STEM careers. By coupling workshops and mentorship, the Molding Melanin Magic program seeks to encourage mentees to serve as mentors along their educational journey, and apply for college and professional school to pursue a career in STEM.

Spiders are sentinel species, organisms that serve to map the bioavailable fraction of contaminants in an ecosystem by retaining their contaminants in their tissues. For example, spiders in the families Tetragnathidae and Araneidae are frequently used as sentinels of mercury contamination of aquatic ecosystems. Spiders are frequently preserved in alcohol prior to contaminant analysis but the impact of contamination on mercury concentrations in spiders has not been assessed. The objective of the present study was to determine the effects of different preservation methods on mercury concentrations in tissues of spiders in the families Tetragnathidae and Araneidae. The spiders were collected along water sources using nets and gloved hands. The Tetragnathids were collected from grassy terrain or a bridge overhanging the water of Lake Weatherford. The araneids were collected from a boat dock overhanging Eagle Mountain Lake. On site, each spider was placed into its respective bottle of varying ethanol or Ziplock's for freezing. Individual spiders were placed into one of three different concentrations of ethanol (100%, 95% , 70%) or frozen. Following about two months of preservation, the spiders were dried and run through the DMA-80 collecting the data for data analysis. Data xxxx *insert conclusion info*

Role of ClpX in the stress response and virulence of Bacillus anthracis: protease or chaperone?
Lillian Wilson, Vuong Do, S.M McGillivray
Department of Biology, Texas Christian University

Anthrax is a lethal infectious disease caused by the bacterial pathogen Bacillus anthracis. Our lab studies the virulence and antibiotic resistance of B. anthracis and we have identified a chromosomal gene clpX, as an important virulence factor, as its loss increases susceptibility to cell-envelope targeting antibiotics such as penicillin, daptomycin, and the antimicrobial peptide LL-37. ClpX is an ATPase that can act autonomously as a chaperone, or with a proteolytic core, ClpP, to degrade proteins. To investigate the mechanism ClpX uses, a plasmid pclpXI264E was designed with a mutation in clpX (I264E) that prevents ClpP binding and inhibits the formation of the ClpXP protease but does not disrupt the chaperone activity of ClpX. We used this to create 4 strains in the unencapsulated Sterne strain: wild-type and ∆clpX containing the empty inducible plasmid pUTE657, complementation plasmid with the non-mutated clpX gene (∆clpX + pclpX), and the mutated plasmid (∆clpX + pclpXI264E). Prior research done on these strains confirmed that ClpX relies on protease activity in antimicrobial stress; however, our goal was to assess its response in other environmental stressors such as acid stress, heat stress, and its virulence in vivo with the Galleria mellonella infection model. We find that that the protease activity of ClpX is important for all of these stresses. These results build on our earlier understanding and demonstrate that formation of the ClpXP protease is critical and any future development of drugs targeting the ClpX system should focus on protease formation rather than chaperone activity.

This research is focused on gaining a better understanding of PKC-epsilon a calcium-dependent protein kinase involved in a wide range of cellular functions including cell proliferation, survival, and apoptosis. The interest in PKC-epsilon derives from the discovery of a de novo mutation in the PKC-epsilon gene in patients suffering from SHORT syndrome. This syndrome is a debilitating disorder characterized by short stature, hyperextensibility, ocular depression, Rieger anomaly, and teething decay. The project involved recapitulating the naturally occurring de novo mutation in vitro as well as determining if other mutations in PKC-epsilon could cause similar disease-state phenotypes. Using a technique known as Site Directed Mutagenesis mutations were introduced into the PKC-epsilon gene and the effects of these mutations on the protein expression were assessed. This mutational analysis will help identify the regions of PKC-epsilon that are vital for its function. This will help elucidate the effect of the same mutations in patients and could help predict the severity of disease. Obtaining a clearer picture of the different regions of the PKC-epsilon protein allows for future studies to focus on successfully fixing these regions when they become damaged and could therefore be used to help patients with SHORT syndrome.

N-terminal acetylation is essential for the stability, activity, and targeting of proteins in eukaryotes. However, most eukaryotic proteins are not acetylated when expressed in bacteria. Therefore, it is of practical significance to control N-terminal acetylation of recombinant proteins in bacteria. RimJ is an N-terminal acetyltransferase (NAT) known to acetylate many recombinant proteins with a narrow substrate specificity in E. coli. This project is aimed to increase the applicability of RimJ for the N-terminal acetylation of a broad range of recombinant proteins.
Based on the AlphaFold-predicted structure of E. coli RimJ, we predicted that six amino acids (Y35, E46, R49, Y106, Y170, and L171) may recognize substrate proteins in the active site. We created RimJ variants, in which one or two of these amino acids are changed to alanine, a small neutral amino acid, so that the active site becomes larger to accommodate substrate proteins containing bigger N-terminal amino acid residues. The RimJ variants were created using site directed mutagenesis, confirmed by DNA sequencing, and co-expressed with Z domain mutants that were not acetylated by the wildtype RimJ. The Z domain mutants were isolated by immobilized metal ion affinity chromatography and analyzed by mass spectrometry for their N-terminal acetylation patterns.