Hydrilla verticillata is an invasive aquatic weed in the United States (U.S.) that has recently
developed resistance to the herbicide fluridone. In this study, we utilized genome walking and
quantitative real-time PCR to investigate the phytoene desaturase (PDS) gene copy number of
hydrilla samples with different ploidy levels. We asked 1) if copy number simply corresponds to the
ploidy level, and 2) if there is increased PDS copy number in resistant populations due to gene
duplication. Using qPCR and microsatellite loci to compare PDS copy number between diploid,
triploid and tetraploid samples, we found that diploid hydrilla from Africa showed higher PDS copy
number than triploid populations from the U.S. The results also indicated that there was no
significant difference in PDS gene copy numbers between the fluridone-resistant and -susceptible
triploid populations. Our study suggests that PDS amplification may not be a mechanism
responsible for fluridone resistance in hydrilla.

Methylmercury (MeHg) is a toxic environmental contaminant found in all waterbodies on Earth. Aquatic emergent insects, such as mosquitoes and midges, can transfer MeHg from waterbodies to terrestrial ecosystems. Terrestrial shoreline spiders consume aquatic emergent insects and become contaminated with MeHg. Methylmercury-contaminated spiders can pose a risk to songbirds that consume terrestrial spiders. Because shoreline spiders have MeHg concentrations that reflect MeHg contamination of nearby aquatic ecosystems and are an important source of MeHg to songbirds, they have been proposed as a biosentinel species that can be used to estimate MeHg contamination of waterbodies. In this study, I used long-jawed orb weavers (Tetragnatha sp.) as a biosentinel species to examine MeHg contamination along the Clear Fork and the West Fork of the Trinity River, Fort Worth, Texas. The objectives of this study were to: 1) evaluate MeHg contamination in long-jawed orb weavers from two forks of the Trinity River, and 2) determine if the concentrations of MeHg in the spiders pose a risk to songbirds that feed on spiders. I collected 101 and 105 spiders along the Clear Fork and the West Fork, respectively. I used a Direct Mercury Analyzer to determine the total Hg concentration of the long-jawed orb weavers. Because MeHg is the primary species of mercury in spider tissues, I used total Hg as a proxy for MeHg. All spiders were contaminated with MeHg, with spiders along the Clear Fork having significantly higher MeHg concentrations than spiders along the West Fork. Methylmercury in spiders increased with spider size along the Clear Fork. Concentrations of MeHg in spiders along the Clear Fork and the West Fork were high enough to pose a risk to the physiology of nestling songbirds that feed on spiders.

Understanding bacterial virulence is important because it provides insight into the molecular basis behind bacterial infections. With the decreased efficacy of antibiotics due to the development of drug resistance, this knowledge could be used to identify specific targets for new pharmacological targets thereby strengthening our arsenal against these pathogens. Currently, our main mechanism by which to evaluate in vivo virulence is the mouse model (Mus musculus). While this model is effective, there are substantial ethical and resource constraints associated with vertebrate use. In order to provide alternative in vivo testing models, this study investigated the invertebrate wax worm larvae, G. mellonella, as an in vivo infection model for B. anthracis. To validate the ability of G. mellonella to discern attenuated bacterial strains, previously identified virulence mutants were constructed and assessed. This model proved capable of distinguishing between virulent and avirulent strains. Next, we tested whether G. mellonella could identify novel virulence mutants. A small collection of transposon mutants was screened for deficits in reactive oxygen species (ROS) survival and iron acquisition using in vitro screens. This yielded 10 attenuated mutants. These mutants were then assessed in G. mellonella and 2 were found to have an in vivo phenotype. These results demonstrate the potential effectiveness of G. mellonella as a future infection model and could increase the efficiency in the identification of novel bacterial virulence mutants.

Dreissena polymorpha, zebra mussels, are an invasive species of freshwater bivalves that have recently spread into bodies of water across North America via the Great Lakes. Zebra mussels are mainly spread throughout the United States by their free-swimming larvae called veligers that are moved from waterbody to waterbody by human boat traffic, attributing to the success of their invasive spread. Once an adult zebra mussel population is established, they proliferate quickly and cause many problems to the ecosystem by their efficient filter feeding abilities. They also cause damage to boating and water treatment equipment by tightly attaching to many hard surfaces. Zebra mussels have recently entered many Texas waterways, indicating that they have possibly adapted to conditions outside of originally expected for a cold water species that are not representative of the Great Lakes region. The focus of this study was to look at various environmental factors which may affect zebra mussel survival and reproduction including temperature and the effects of a copper-based molluscicide, EarthTec QZ, as a potential mechanism of control. Zebra mussel survival and reproductive success were examined in various experiments to gain an overall understanding of the effects at all zebra mussel life stages.

Methylmercury (MeHg) is an aquatic contaminant that can be transferred to terrestrial predators by emergent aquatic insects such as odonates (damselflies and dragonflies). We observed the effects of time on odonate-mediated MeHg flux (calculated as emergent odonate biomass MeHg concentration) in 20 experimental ponds and the potential risk to nestling red-winged blackbirds (Agelaius phoeniceus) posed by consuming MeHg-contaminated odonates. Emergent odonates were collected weekly from ponds containing four emergent traps per pond over an 9-mo period (February–October 2017). The MeHg flux from damselflies, aeshnid dragonflies, and libellulid dragonflies began in March and peaked in May, June, and July, respectively, and then declined throughout the rest of the summer. Nesting of red-winged blackbirds overlapped with peak odonate emergence and odonate-mediated MeHg flux. Because their diet can be dominated by damselflies and dragonflies, we tested the hypothesis that MeHg-contaminated odonates may pose a health risk to nestling red-winged blackbirds. Concentrations of MeHg in odonates exceeded wildlife values (the minimum odonate MeHg concentrations causing physiologically significant doses in consumers) for nestlings, suggesting that MeHg-contaminated odonates can pose a health risk to nestling red-winged blackbird.