This study explored the extent to which nostalgia proneness (a sentimental longing for the past) is associated with attitudes towards intimate partner violence (IPV). Research has found that individuals who report more conflict in their romantic relationships also report being more nostalgic for their own and for their relationships’ past. If nostalgia is related to more conflict in relationships, then it may also be related to greater acceptance of IPV. In this study, a sample of 142 participants completed measures of self-relevant nostalgia, relationship-relevant nostalgia, and attitudes toward IPV (using it and enduring it), and relationship outcomes (e.g., optimism, satisfaction, commitment). Results showed positive correlations between nostalgia (self-oriented and relationship-centered) and self-use of IPV (both using it and enduring it). These preliminary results suggest that a sentimental longing for the past is associated with endorsement of IPV use, however, other unexplored personality variables such as attachment style may moderate these associations. Future work should explore these findings in experimental and longitudinal designs.

Investigating how people regulate their learning is important because study decisions can impact actual learning. Compared to younger adults, older adults often show age-related deficits in memory. This deficit may be because older adults are less effective at regulating their learning. One factor that can influence memory is the valence of information. Prior research has established that older and younger adults are more likely to recall emotional information compared to neutral information and also predict that emotional information will be better remembered relative to neutral information (e.g., Tauber & Dunlosky, 2012). It is unclear how both age groups regulate their learning of emotional and neutral information. Investigating this issue, older and younger adults studied words that were positive (e.g., circus), negative (e.g., snake), or neutral (e.g., fork). Participants regulated their learning by self-pacing their study (Experiment 1) or by selecting half of the words to restudy (Experiment 2). After studying each word, participants predicted the likelihood of remembering it on a scale of 0% (will not remember) to 100% (will remember). Finally, participants took a free-recall test. Consistent with prior research, both age groups demonstrated higher predicted and actual memory for emotional information relative to neutral. Importantly, both age groups’ self-paced study times did not differ for emotional and neutral information. In contrast, both age groups restudied neutral words more frequently than emotional words. Thus, when participants were forced to strategize their learning, both age groups made good study decisions, prioritizing neutral information at the expense of emotional information.

For my SERC grant proposal, I studied the effect of auricular vagus nerve stimulation (aVNS) on learning in adults with dyslexia. The vagus nerve is a cranial nerve that, when stimulated, initiates the release of two neurotransmitters (NT’s) that are important in learning and memory (norepinephrine and acetylcholine). When a stimulus is presented at the same time as vagus nerve stimulation, this increases neural plasticity for the paired item. We have already tested this approach on typically-developing adults using the auricular branch of the vagus nerve, which runs through the ear and can be stimulated non-invasively. During this intervention, timed bursts of electrical stimulation were delivered while the participant learned novel letter-to-sound correspondences for Hebrew letters with the goal of increasing recall and automaticity. We have already found significant improvements in letter recognition, reading speed, and nonword decoding in typically-reading participants receiving stimulation compared to those in control groups. Our ultimate goal is to help children with dyslexia read more fluently. In the first step towards this goal, we enrolled a group of adult participants with dyslexia who received 10 days of Hebrew orthography training paired with aVNS. Participants were evaluated at four timepoints to monitor learning and compare progress with other groups: at day 1, halfway through training, at the end of training, and 3 weeks after training ended. We measured letter recognition, letter-to-sound fluency, and decoding at each time point. We will present our preliminary findings at SRS and discuss future directions.

Early life stress has shown to be related to an increased preference for smaller, more quickly acquired rewards over larger, delayed rewards—or an inability to delay gratification—a fundamental component of impulsivity. Beyond this, impulsivity is also characterized by difficulty concentrating and exercising self-control and has been found to significantly impact learning and memory. Specifically, in children, higher impulsivity is associated with greater learning difficulties, such as with reading. Previous research has also shown that adults with higher levels of inflammation portray higher impulsivity. The current study aims to investigate the relationship between impulsivity, inflammation, and childhood environmental conditions within children between the ages of 3-17. Saliva samples were collected from 248 children visiting the Fort Worth Museum of Science and History in order to measure current levels of proinflammatory cytokines, which indicate immune activation. Children then participated in a series of tasks that measured their ability to concentrate, learn to inhibit their responses, and delay gratification, while background and demographic information was collected from their parents. Results will reveal whether children growing up in stressful environments also have higher levels of inflammation and impulsivity.

Attitude Representation Theory (Lord & Lepper, 1999) asserts that individuals evaluate attitudes based on a subset of associations. As this subset of associations varies, attitudes can vary as well. Previous research demonstrated that people can mistake self-generated information for provided information, through source monitoring errors (Johnson, Hashtroudi, & Lindsay, 1993), after extrapolating beyond the information given (Lu, 2015). We sought to apply ART and cognitive tasks (e.g., extrapolation, embellishment, and validity) by having participants judge the actions of fictitious groups. Although these groups are fictitious, they allude to current political viewpoints. We tested the effects of extrapolation (thinking about additional attributes of a target group; Experiment 1), embellishment (convincing a friend not to join the target group; Experiment 2), and biased assimilation (testing truth and validity of sources; Experiment 3) on polarization of moral judgments. Compared to a control group, embellishment polarized negative attitudes toward the group. The current set of studies could shed some insight about how people view issues, self-radicalize judgments, and understand thoughts of lone-wolf terrorists.

There are many benefits for children to receive music education. Research shows that note reading and music playing skills are positively correlated with cognitive development, motor proficiency, and self-esteem (Bilhartz, Bruhn, & Olson, 1999; Costa-Giomi, 2004; Schellenberg, 2004). The purpose of this study was to evaluate the use of equivalence-based instruction (EBI) to teach elements of music to children who have no music background in the age range of 4-7 years. The children first learned to relate the name of a note (e.g. “quarter”, “half”) to the length of the note as well as a picture of the note. They were also taught to name the lengths and names of sequences of notes (e.g. “quarter, quarter, half”). In the second part of this study, the children learned letter names of notes (e.g., “A”), keyboard placement, and what finger (e.g. thumb) goes with each letter name and piano key. Finally, we tested for emergent relations from part one and part two. Date collection in progress, but we predict to see emergence of relations between what was taught in part one and what was taught in part two. For example, when told “play this sequence on A,” participants will be able to use the correct finger on the correct piano key to play the notes in the sequence at the correct lengths.

Since the 1920s, it has been recognized that nonhuman animals are capable of forming expectations about rewards and exhibit emotional responses when those expectations are violated—when obtained rewards have lower value than expected rewards. Our lab utilizes a rodent model for coping with unexpected reward loss with a specific interest in furthering our understanding of the underlying neural correlates. Frustration effects in rats are commonly and reliably produced using the consummatory successive negative contrast (cSNC) procedure, where rats are given access to a highly preferred 32% sucrose solution followed by an unexpected downshifted to 4% sucrose. Such surprising nonreward leads to a suppression of behavior compared to a control group that always received the less-preferred, 4% sucrose solution. Studies involving neurological manipulation indicate that permanent lesion or reversible deactivation of the central amygdala (CeA) and the basolateral amygdala (BLA) eliminate the cSNC effect. While these studies are important for identifying key structures, they provide little information about the underlying circuitry. The present research examined the role of the neural pathway between the BLA and CeA in the cSNC task using a chemogenetic approach known as Designer Receptor Exclusively Activated by Designer Drugs (DREADDs). Inhibitory DREADDs are intracranially infused into the key structures and later activated by intraperitoneal injections of clozapine N-oxide (CNO). Both groups of rats received unilateral inactivation of the BLA and CeA. The experimental (contralateral) group has one functioning area in each hemisphere, a procedure that disrupts communication between the two areas. The control (ipsilateral) group has one hemisphere disrupted while the other is left intact. Preliminary results indicate a disconnecting the BLA-CeA pathway reduces the cSNC effect in contralateral rats compared to ipsilateral rats. The BLA-CeA pathway is necessary to respond to surprising nonreward. These results add to the hypothesized model of circuity underlying unexpected reward loss in mammals. Because the amygdala circuitry is highly conserved across species, these results inform us about the neural circuitry engaged by similar instances of frustrative nonreward in the human brain.

According to Terror Management Theory, religious people are motivated to cling to their religious beliefs when reminded of their death. Quest motivated religious people, however, value doubt in their beliefs and are uncertain about the validity of their religion. The purpose of the present work was to examine medical decision making among individuals who actively question their religious beliefs when death concerns are salient. Specifically, we found that individuals who are quest motivated reject traditional faith healing in favor of more scientific medical practices. These effects were exacerbated by death awareness. These findings support previous research suggesting that quest individuals are motivated to embrace their religious doubts in when faced with existential concerns.

Hepatitis C Virus is estimated to infect 3% of the world’s population and is transmitted by contaminated blood. HCV can be asymptomatic or lead to cirrhosis of the liver, liver cancer or hepatocellular carcinoma. Understanding the virus life cycle and the viral proteins generated by HCV will help generate new mechanisms of inhibition of the virus. HCV produces 10 viral proteins when it infects hepatocytes that increase the reproduction ability of the virus. The anti-viral response of the body uses transcription factors such as ATF-2, NFKB, and IRF-3 that translocated into the nucleus and bind to the interferon gene that produces interferon to alert the body of a viral infection. HCV viral protein NS3/4A acts as protease to cleave the polycistronic strand of viral proteins made by HCV and is known to inhibit IRF-3 movement into the nucleus to inhibit the production of interferon. HCV viral protein NS5A is known to inhibit the movement of transcription factor NFKB into the nucleus, thus inhibiting the anti-viral response. We are interested to see if NS3/4A inhibits the anti-viral response by blocking the movement of both transcription factors IRF-3 and NFKB into the nucleus.

Psychological stress afflicts a considerable portion of the world’s population, and is linked, as both a risk factor and potential contributor, to dementia-related brain dysfunction in diseases such as Alzheimer’s disease (AD). The brain dysfunction in AD is marked by an increase in Amyloid-beta, the protein responsible for plaque deposition in the brain. The present study aimed to explore alterations in the production of amyloid-beta in response to stress and inflammation. Specifically, we were interested in social isolation stress- and inflammation-induced differences in cognition and amyloid-beta production in male and female mice. Mice were subjected to acute social isolation (6 days) and chronic isolation (28 days) or control group housing. Results revealed that exposure to both acute and social isolation can lead to an exacerbated inflammatory response to lipopolysaccharide (LPS). Subsequently, we examined if the stressors altered amyloid-beta production following the same inflammatory stimulus. Animals received LPS or saline injections once per day for seven consecutive days after the completion of either stress protocol or group housing. The contextual fear conditioning paradigm (CFC) was utilized to assess cognition. Brain tissue extractions were performed to quantify amyloid-beta protein levels. It is hypothesized that isolated animals will demonstrate cognitive deficits in CFC as well as increased brain amyloid-beta following LPS injections.

Hydrilla verticillata is an invasive aquatic weed in the United States (U.S.) that has recently
developed resistance to the herbicide fluridone. In this study, we utilized genome walking and
quantitative real-time PCR to investigate the phytoene desaturase (PDS) gene copy number of
hydrilla samples with different ploidy levels. We asked 1) if copy number simply corresponds to the
ploidy level, and 2) if there is increased PDS copy number in resistant populations due to gene
duplication. Using qPCR and microsatellite loci to compare PDS copy number between diploid,
triploid and tetraploid samples, we found that diploid hydrilla from Africa showed higher PDS copy
number than triploid populations from the U.S. The results also indicated that there was no
significant difference in PDS gene copy numbers between the fluridone-resistant and -susceptible
triploid populations. Our study suggests that PDS amplification may not be a mechanism
responsible for fluridone resistance in hydrilla.

Methylmercury (MeHg) is a toxic environmental contaminant found in all waterbodies on Earth. Aquatic emergent insects, such as mosquitoes and midges, can transfer MeHg from waterbodies to terrestrial ecosystems. Terrestrial shoreline spiders consume aquatic emergent insects and become contaminated with MeHg. Methylmercury-contaminated spiders can pose a risk to songbirds that consume terrestrial spiders. Because shoreline spiders have MeHg concentrations that reflect MeHg contamination of nearby aquatic ecosystems and are an important source of MeHg to songbirds, they have been proposed as a biosentinel species that can be used to estimate MeHg contamination of waterbodies. In this study, I used long-jawed orb weavers (Tetragnatha sp.) as a biosentinel species to examine MeHg contamination along the Clear Fork and the West Fork of the Trinity River, Fort Worth, Texas. The objectives of this study were to: 1) evaluate MeHg contamination in long-jawed orb weavers from two forks of the Trinity River, and 2) determine if the concentrations of MeHg in the spiders pose a risk to songbirds that feed on spiders. I collected 101 and 105 spiders along the Clear Fork and the West Fork, respectively. I used a Direct Mercury Analyzer to determine the total Hg concentration of the long-jawed orb weavers. Because MeHg is the primary species of mercury in spider tissues, I used total Hg as a proxy for MeHg. All spiders were contaminated with MeHg, with spiders along the Clear Fork having significantly higher MeHg concentrations than spiders along the West Fork. Methylmercury in spiders increased with spider size along the Clear Fork. Concentrations of MeHg in spiders along the Clear Fork and the West Fork were high enough to pose a risk to the physiology of nestling songbirds that feed on spiders.

Methylmercury (MeHg) is an aquatic contaminant that can be transferred to terrestrial predators by emergent aquatic insects such as odonates (damselflies and dragonflies). We observed the effects of time on odonate-mediated MeHg flux (calculated as emergent odonate biomass MeHg concentration) in 20 experimental ponds and the potential risk to nestling red-winged blackbirds (Agelaius phoeniceus) posed by consuming MeHg-contaminated odonates. Emergent odonates were collected weekly from ponds containing four emergent traps per pond over an 9-mo period (February–October 2017). The MeHg flux from damselflies, aeshnid dragonflies, and libellulid dragonflies began in March and peaked in May, June, and July, respectively, and then declined throughout the rest of the summer. Nesting of red-winged blackbirds overlapped with peak odonate emergence and odonate-mediated MeHg flux. Because their diet can be dominated by damselflies and dragonflies, we tested the hypothesis that MeHg-contaminated odonates may pose a health risk to nestling red-winged blackbirds. Concentrations of MeHg in odonates exceeded wildlife values (the minimum odonate MeHg concentrations causing physiologically significant doses in consumers) for nestlings, suggesting that MeHg-contaminated odonates can pose a health risk to nestling red-winged blackbird.

Alzheimer’s Disease (AD) is a neurodegenerative disease that is characterized by deficits in learning and memory. AD pathology is associated with neuronal death through the accumulation of amyloid beta (Aβ) plaques in the synapses. Our lab has previously demonstrated that Lipopolysaccharide (LPS), a component of gram-negative bacteria, induces an inflammatory response that increases Aβ found in the brain. Dendritic spines are projections on dendrites that may or may not be synapsing with an axon. Previous research indicates that there is a correlation between the number of properly functioning synapses and the number of dendritic spines. In this study, LPS was administered to induce inflammation, stimulating Aβ production. We then quantified dendritic spine density in order to compare dendritic spine density in the hippocampus of both LPS- and saline-treated groups. Contrary to our hypothesis, we saw a non-significant increase in dendritic spine density following LPS treatment, when compared to saline controls.

There has been mounting concern from both scientists and the public regarding the presence and biological effects of emerging contaminants (ECs) in the environment. ECs can be defined as contaminants that are not currently subject to routine monitoring programs or regulatory standards, but that have the potential to cause adverse environmental or human health effects. These pollutants are being found in increasing levels in aquatic environments, and as such, the possible health impacts of these contaminants have become a growing focus of scientific research. Some classes of ECs, especially those that disrupt neurological development or thyroid hormone levels, have the potential to alter the growth, development, and function of the eyes. For many organisms, eyesight is crucial to survival as it allows them to avoid danger, obtain food, and perform many other important activities. However, reliable methods for testing the effects of ECs on vision are scarce, so the full impact of many ECs remains unknown. As such, this project aimed to determine dependable ways to measure visual development and function in the fathead minnow, a small fish frequently used to screen for chemical toxicity and adverse effects. We found that the feeding assay was a straightforward and promising option for measuring vision because it estimated the average prey capture ability of a group in a relatively short amount of time. We also found that the optomotor assay, while compelling, presented no significant differences between groups for the variables tested. However, there were practical differences observed throughout the trials, indicating that although the assay is complex, further testing and development could transform it into a reliable source of data.

Zebra and Quagga mussels are aquatic and highly invasive freshwater bivalve molluscs native to Eurasia. They have spread at an exponential rate into bodies of water throughout the country by means of our interconnected waterway. Prior analysis of their distribution has determined a consistent global pattern in which a population of zebra mussels initially invades a body of water and subsequently, a population of quagga mussels is established in the same region. Despite differential habitat preferences, both species have been found to live and reproduce in the same location. Since both species exhibit broadcast spawning as a reproductive mechanism, the potential for hybridization exists; this potential was analyzed via evaluating the initial fertilization and early embryonic cleavage stages required for production of viable hybrid offspring. A series of hybridization crosses were performed and compared against a control. Fertilization events observed and analyzed included motility and chemotaxis, the acrosome reaction, sperm binding and entry into the egg cytoplasm, and finally cleavage and early development. Inability to produce viable offspring suggests a hybridization-block has been established between the two species at the level of fertilization or early development.

Some classes of endocrine disrupting compounds in the environment have the ability to alter thyroid function. Such thyroid disrupting compounds are known to influence growth and development, but recent studies suggest that thyroid disruption can also have adverse effects on reproduction. A recent study in the Jeffries lab demonstrated that early-life stage thyroid disruption caused decreased reproductive output in fathead minnows (Pimephales promelas), even after a prolonged period of depuration. However, the mechanisms connecting early life stage thyroid disruption to altered reproduction during adulthood remain elusive. This study sought to determine whether alterations in reproductive success following thyroid disruption were a result of male or female reproductive performance in an effort to narrow potential mechanisms by which thyroid disrupting compounds alter reproduction. The results of this study bring insight to the underlying cause of decreased reproductive output following thyroid inhibition.

Carnivorous plants occupy nutrient-poor soils and have evolved traits that allow them to obtain nutrients by capturing and digesting insects. The pale pitcher plant, Sarracenia alata, uses passive pitfall traps to capture their insect prey. Although studies have examined prey composition for S. alata, it is unknown whether this species is selective in prey capture or whether it captures insects in proportion to their abundance in the environment. The purpose of this study was to compare prey capture of S. alata pitchers with the available insects to determine whether this species is selective in prey capture. The available insects were sampled using artificial sticky traps in the vicinity of the pitchers. The insects in the study were identified first to the taxonomic level of order and then further identified to "morphospecies" as a means of examining preference on a finer scale. The relative proportions of insects in specific orders differed between artificial traps and plants. Although dipterans were a major component of capture in both artificial traps and plants, the relative proportions of morphospecies differed between the two. These results support the hypothesis that S. alata is selective in its prey capture, but further studies are needed that use different methods of measuring the available insects in order to avoid potential bias.

Our team will answer the question how Penicillium mold grows in a microgravity environment versus Earth’s gravity. This question answers or sparks several other questions such as is it a viable solution for some antibiotics in space or how do antibiotics like penicillin work in the body in space. Will it grow more or will it be the same or maybe grow less? The purpose of our experiment is to provide a viable solution to some bacterial infections in space. Bacteria in space tends to act more violently so maybe good bacteria or mold will act more furiously to kill those bacteria. Our hypothesis is that it will grow better. This is based off of the fact that in an earlier SSEP experiment the polymers absorbed more water. Which might be the same for organisms like mold so it would make it easier to absorb water. Plus with lower gravity organisms tend to grow larger at least that is many scientist hypotheses. So since there is practically no major gravity or forces in space may be the mold will grow larger than usual. Our group believes this based on the fact that we have researched.

Our experiment is about diabetes and Humalog synthetic insulin crystallization in a microgravity environment. We feel like this is a good experiment to design because we could find out if there is a way to prevent crystallization of insulin, especially if we understand how it happens in microgravity. When insulin crystallizes, the bacteria that usually makes it viable stops working. This would cause it to be ineffective for patients in dire need of this medication. To complete this experiment we are going to keep the insulin in a type 1 FME at the International space station (ISS) at above 65℉ to see if it crystallizes within a certain amount time. We will keep the experiment refrigerated at or below 40℉ during transportation to the ISS and again on arrival back to Earth’s gravity. Refrigeration slows the crystallization growth and this is how it is stored on Earth. Keeping our experiment refrigerated during transportation is an important step because the insulin crystallization growth should only be measured while in microgravity. We will be conducting the same experiment, using the same time frame and refrigeration needs before and after, for our earth bound experiment.

Our experiment is how well will a hornwort plant purify polluted water in microgravity. We will see how it will purify at the same rate as it does in full gravity. We chose this plant because they can purify water and they grow at a fast rate. This will help astronauts because if they run out of water they can grow hornwort even if the only water they have is polluted. Also, it will help them to have purified water if their water system breaks down. The hornwort plant will be growing on the way from Earth to the ISS. The experiment will be purifying the polluted water in microgravity for 5-6 days. Then the formalin will be added to the plant to stop its growth and preserve the sample. We are polluting the water with Cyanobacteria, which is more commonly known as blue green algae. We will know it has worked if the polluted water has become purified after it has been tested.

Previous studies, including those in the Jeffries lab, have shown that female animals are able to fight and survive infection better than males. However, the underlying cause of this difference remains unclear. Because many differences between males and females are due to differences in sex steroid hormone (e.g., estrogen, testosterone, etc.) concentrations, it is possible that differences in immune function are also due to such differences in hormone levels. The objective of this study is to uncover the role of sex steroid hormones in the immune response of fathead minnows (Pimephales promelas). Because females exhibit better pathogen resistance than males, it is hypothesized that estrogen (a “female” hormone) enhances immune system function. The results of this study provides insight into the potential crosstalk between the reproductive and immune systems, as well as a better understanding of the role of sex hormones in the organism.

The fathead minnow (Pimephales promelas), a small fish model often used to screen for reproductive endocrine disrupting compounds, has recently been used by some investigators to screen for chemicals with thyroid disrupting capabilities. However, it is uncertain how known thyroid disruptors affect various markers of thyroid disruption in this species. This study aimed to fill this gap in knowledge by assessing the sensitivity of endpoints known to be responsive to thyroid disruption in other closely-related species in larval fathead minnows. In addition, we sought to uncover how the timing and length of exposure influenced the response of these endpoints. To accomplish these objectives, larval fathead minnows were exposed to various doses of propylthiouracil (PTU; a known thyroid disruptor) and thyroxine (T4; a known thyroid stimulant) for 35 days. Several metrics indicative of alterations in thyroid hormone status (e.g., thyroid related gene expression, growth, thyroid cell follicular height, etc.) were measured on day 7, 21, and 35. The results of this study provide valuable information that can be utilized in developing fathead minnow thyroid disrupting chemical screening assays.

Cancer is the second leading cause of death and will directly affect approximately 40% of the people in the United States over the course of their life. Chemotherapy has been shown to be an effective therapeutic strategy, but it lacks specificity, resulting in a multitude of negative side effects. Targeted therapies such as Herceptin, Iressa, and Nivolumab have shown increased effectiveness against cancer by attacking specific molecules in the target cell. For example, Herceptin inhibits the HER2 protein, which is overproduced in some breast cancer cells, and stops cell division. Biotin is an innate coenzyme for carbohydrate, lipid and protein metabolism. Certain cancer types overexpress biotin transporters on the surface of each cancer cell in order to increase biotin absorption necessary for metabolic processes. Furthermore, the intracellular environment in cancer cells is more reducing compared to non-cancer cells due to increased metabolism. Ferrocene is an iron-based organometallic molecule that has been shown to generate reactive oxygen species (ROS) in the reducing environment of cancer cells. Given that certain cancer cells absorb biotin with a higher efficiency, we hypothesize that linking biotin to ferrocene will increase the efficiency of ferrocene entering the cell and result in selective cancer cell death. Therefore, we have produced a library of biotin-ferrocene conjugates to selectively target cancer cell lines that over express biotin receptor sites. Experiments were conducted utilizing ferrocene and a variety of ferrocene-biotin conjugates (C1, C2, 2) in both cancer (MCF-7) and noncancer (HEK 293) cell lines in order to compare the relative toxicity between compounds.

The Texas horned lizard (Phrynosoma cornutum) has always been believed to be an ant specialist, especially on harvester ants. However, a population of horned lizards in south Texas seem to have a more diverse diet consisting of other insects and arachnids. The goal of this project is to build a DNA library of order Coleoptera (beetles) that are preyed upon by these horned lizards. This DNA library will be compared to DNA extracted from horned lizard scat so that we can identify which species of beetles these lizards are eating. For this process, I isolated DNA from 244 beetles collected in pit fall traps from Kenedy and Karnes City, amplified the cytochrome oxidase I (COI) gene, and sequenced it. I compared the processed sequences to those available on GenBank and BOLD (Barcode of Life Database) to identify the species of beetle.