Reactive oxygen species (ROS) are byproducts of normal cellular metabolism and play important roles in cell signaling and immune defense. However, when their production exceeds the cell’s antioxidant capacity, ROS accumulation leads to oxidative stress, damaging proteins, lipids, and DNA. In the brain, this oxidative imbalance has been closely linked to the development and progression of neurodegenerative diseases like Alzheimer’s. Under normal conditions, superoxide dismutase (SOD) enzymes play a key role in protecting cells by breaking down harmful superoxide radicals. Yet, reduced SOD activity and impaired regulation have been consistently observed in patients with neurodegeneration, including Alzheimer’s disease. Small-molecule mimics of SOD, therefore, represent a promising therapeutic approach. In this study, we evaluate an expanded library of tetra-aza macrocyclic ligands chelating either copper or manganese metals. Mechanistic analysis reveals how structural modifications to the macrocyclic ring, particularly R-group substitutions that alter steric environment and electronic properties, directly influence catalytic reactivity and stability. Evaluation of Cu- and Mn-based complexes highlights distinct trends in activity and identifies structural motifs that enhance SOD-like function. These findings provide design principles for developing antioxidant therapeutics targeting oxidative stress.

“Superfrog Science: Experiments for at Home and in the Classroom” is a creative endeavor that encourages young scientists to get curious about science and help learn a variety of chemistry concepts. This book is a visual representation of the importance of exploring the bounds of creativity in science. Join Superfrog as he goes on a learning adventure conducting science experiments and using the scientific method to deepen his knowledge about chemistry, making it easy to learn by conducting each experiment with easy-to-follow comic panels. This book strives to make science experiments accessible, affordable, and fun. It is perfect for encouraging hands-on learning, with in-depth explanations of the “how” and “why” of these experiments. Superfrog asks discussion questions and provides variations of the experiments to get his students to think about cause-and-effect and variable manipulation when it comes to the scientific process, as well as encourage collaboration with his peers. The educational activities featured are made for scientific discovery inside and outside the classroom. Make chemistry fun and easy with Superfrog!

Riboswitches are structured RNA elements that regulate gene expression through ligand-induced conformational changes and provide a platform for engineering cell-based biosensors. By coupling aptamers to reporter genes, synthetic riboswitches enable small-molecule–dependent detection of clinically relevant metabolites. This study focuses on sarcosine, associated with prostate cancer progression, and urate, linked to gout. Two sarcosine-responsive candidates were evaluated in E. coli using β-galactosidase and GFP reporter systems. Although construct integrity was confirmed, neither candidate demonstrated ligand-dependent activation in CDR or minimal media, suggesting insufficient regulatory activity under tested conditions. In parallel, a urate-responsive riboswitch library underwent dual selection with chloramphenicol resistance for positive selection and 5-fluorouracil counterselection for negative selection. After multiple selection rounds and screening of 192 colonies, no urate-specific variants were identified. Increasing chloramphenicol concentration to strengthen positive selection similarly yielded no hits. Future work will focus on further increasing both positive and negative selection intensity to enhance enrichment of functional variants and improve development of RNA-based biosensors for accessible metabolite detection. Additionally, future efforts will explore the adenine riboswitch library as a potential platform for developing novel biomarker detection systems.

Nitric oxide (NO) is a gaseous free-radical 2° messenger with a physiological half-life of 3-5 seconds. Overexpression of the cytoprotective NO can lead to high concentrations of cytotoxic peroxynitrite (OONO^-), causing nitroxidative stress. Studies have shown that nitroxidative stress can be implicated as an etiology of several inflammatory diseases, such as Alzheimer’s Disease (AD) or Parkinson’s Disease (PD). A solution to counter nitroxidative stress is the biomimicry of the enzyme Nitric Oxide Dioxygenase (NOD). The enzymic activity of NOD relies on a heme active site, where excess NO is scavenged to produce nitrate (NO_3^-), a less potent oxidant. Several groups have successfully mimicked this activity; however, it has been restricted to water-insoluble, large molecules (porphyrin rings). While other antioxidant enzymes such as Superoxide Dismutase and Catalase have been successfully mimicked with water-soluble, metal-centered, non-heme scaffolds, to date, there have been no reports of water-soluble non-heme mimics of NOD activity. It is the Green Group’s goal to explore the possibility of developing a molecule capable of NOD enzymic activity. Therefore, theoretical feasibility of this reaction was explored using Density Functional Theory (DFT) as well as Conformer-Rotamer Ensemble Sampling Tool (CREST). Current data shows that based on an energy screening of several simple-to-complex tetra-aza small molecules, the reaction is successful both in gas phase and in water (implicit and explicit solvation). Additionally, computational intermediate spin states have, so far, matched those reported experimentally. Energy diagrams were then proposed based on the most stable ground state energies of structural intermediates. This data provides, for the first time, a new perspective on the possibility of the successful biomimicry of NOD with non-heme, water-soluble, tetra-aza small molecules.

Interactive Querying and Visualization of Solar Events

Author: Thu My Banh, Cathy Nguyen, Pandey Chetraj

Access to structured solar flare event data is essential for space weather (SWx) research, operational analysis, and machine learning applications. While the solar flare event archive maintained by the Lockheed Martin Solar and Astrophysics Laboratory (LMSAL) provides a widely used curated record of flare activity, the archive is primarily accessible through static web interfaces rather than a programmable query system. This makes automated filtering, dataset generation, and large-scale analysis difficult for researchers. To address this limitation, we developed a full-stack web application that provides programmatic access to LMSAL solar flare event records through a queryable API. A Python-based data ingestion pipeline retrieves and deduplicates event information from LMSAL’s rolling snapshot archive and stores it in a structured format. A FastAPI backend exposes endpoints that allow users to filter events by date range and GOES flare classification, enabling rapid dataset generation for analysis workflows. The frontend, implemented in React, allows users to query the event catalog, visualize results in a structured table, and export filtered datasets as CSV or JSON files. To improve data reliability and context, the system cross-references LMSAL event records with NOAA solar flare catalogs, allowing users to compare event metadata across independent data sources. Additionally, the application integrates with the Helioviewer API to display solar imagery corresponding to each event, with derived heliographic positions overlaid onto the solar disk to provide spatial context. The resulting system provides a lightweight platform for exploring, querying, and exporting solar flare event data, lowering the barrier to accessing operational flare records and facilitating dataset generation for space weather analysis and predictive modeling.

Large language models (LLMs) are increasingly framed as force multipliers for cyberattacks, yet most existing evaluations focus on isolated artifact generation rather than the construction and execution of full offensive workflows. This paper presents a controlled empirical study of LLM-assisted cyberattack construction across multiple representative attack classes, including automated SQL injection exploitation, spyware assembly, reverse shell establishment, and denial-of-service traffic generation. We evaluate several contemporary models—including ChatGPT-4o, ChatGPT-5.2, ChatGPT-5.1-instant, Claude Sonnet 4.6, and Gemini 3—within fully sandboxed virtualized environments, treating each model strictly as an advisory system embedded within a human-driven workflow.

Our experimental design decomposes attacks into staged operational workflows encompassing reconnaissance, payload generation, system integration, troubleshooting, and persistence. This structure enables systematic analysis of where automation succeeds or fails during real execution rather than relying on single-shot demonstrations. Across scenarios, LLMs consistently reduce effort for localized technical tasks such as command syntax recall, tool configuration, payload scaffolding, and procedural troubleshooting. However, reliable end-to-end attack execution remains limited. SQL injection automation succeeds primarily when established tools encapsulate complex orchestration, while more complex scenarios such as spyware assembly fail at system-level integration, environment-specific dependency resolution, and evasion of host defenses.

Across models and attack classes, automation consistently breaks at environment-dependent boundaries requiring global reasoning, state awareness, and cross-stage workflow coordination. These findings suggest that contemporary LLMs do not autonomously execute cyberattacks but instead function as workflow accelerators that lower the expertise threshold required to operationalize existing offensive techniques. This capability-boundary perspective provides a more realistic foundation for threat modeling, defensive planning, and future evaluation of AI-assisted cybersecurity risks.

Medical students lack effective tools for developing clinical reasoning, as most resources emphasize memorization rather than decision-making. DiseaseQuest is an AI-powered, gamified platform that addresses this gap through realistic patient simulations and decision-based scenarios. It enables students to work through complete clinical cases using interactive, patient-centered dialogue. Supported by a multi-agent framework, the platform provides adaptive guidance, diagnostic feedback, and personalized evaluations. By promoting active learning and problem-solving, DiseaseQuest offers a transformative approach that replaces passive study with immersive, hands-on practice, helping students strengthen diagnostic thinking and better prepare for real-world clinical decision-making.

As obesity continues to rise in the United States, bariatric surgery has become as increasingly common medical intervention to support significant and sustained weight loss. However, the procedure presents challenges, as patients must adopt strict dietary guidelines, develop consistent meal tracking habits, and maintain long-term lifestyle changes. Existing weight-loss applications fail to address the unique nutritional requirements of bariatric patients, which include surgery-specific restrictions, medical conditions, personal preference in food, and individualized lifestyle factors. Along with that, they lack integrated long-term monitoring tools that allow healthcare providers to effectively track patient progress and adherence after surgery. This senior design project presents a prototype mobile application developed from scratch to support patients throughout the bariatric journey. The application integrates AI-driven personalization to generate tailored daily nutritional guidance, adapt to individual health data, and provide meal tracking support. In addition, the platform centralizes patient data for healthcare providers, improving long-term monitoring, increasing tracking accuracy, and reducing manual workload. By combining personalized patient support with provider-facing analytics, this solution aims to enhance postoperative adherence and improve long-term surgical outcomes.

The Brazos Safety Systems Weather Application provides insight into weather conditions that affect aviation operations. Weather plays a critical role in flight safety, and understanding conditions during flight operations can help explain incidents or risky behavior that occur. The application integrates aviation weather data sources, including METAR reports and radar imagery, to visualize weather conditions around airports and during historical flights. Users can upload flight records and review the weather conditions associated with those flights through the application. By presenting aviation weather data in a centralized and accessible format, the application supports post-flight analysis and helps identify weather-related factors associated with flight incidents. The goal is to provide insights that assist in understanding past flight conditions and help prevent similar issues in future aviation operations.

Solar flares are among the most significant drivers of space-weather disturbances, motivating ongoing efforts to develop reliable forecasting methods from solar observations. The Solar Dynamics Observatory continuously produces high-resolution full-disk solar imagery used for monitoring solar activity. These observations have enabled substantial progress in machine learning–based flare prediction; however, most models remain confined to research studies, with limited deployment in operational systems that support continuous forecasting and systematic performance validation. This work presents a lightweight operational framework for near-real-time solar flare forecasting built around machine learning models proposed in the literature. The system retrieves full-disk solar imagery from the Helioviewer API, performs automated preprocessing, and generates predictions using a convolutional neural network–based forecasting model. Predictions and corresponding observations are stored to enable periodic forecast verification using standard performance metrics, allowing model performance to be monitored over time and potential prediction drift to be identified. The framework is implemented as an interactive application using Streamlit, providing an integrated interface for automated data ingestion, near-real-time inference, and ongoing model evaluation. The proposed system enables continuous monitoring of solar flare forecasts while providing a practical framework for tracking model performance and detecting prediction drift in operational settings.