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COSC2018HENDRICK21849 COSC

Analysis of Artificial Intelligence Techniques for Konane

Type: Undergraduate
Author(s): Kaitlin Hendrick Computer Science
Advisor(s): Michael Scherger Computer Science

This research analyzes artificial intelligence techniques for Konane. The game Konane, also known as Hawaiian checkers, is a two-player, zero-sum strategy board game ideally suited for this research. The game ends when a player does not have a move in which they can capture an opponent’s piece. In order to have a successful strategy, a player must consider many future possibilities. For this reason, this project compares computing agents that use informed and uninformed searching algorithms. We focus our investigation on the effectiveness of the minimax and minimax with alpha-beta pruning algorithms. By altering several variables, specifically the cutoff depth for searching the game tree, we begin to see varying levels of success from the competing computing agents. The outcome of this research will be an analysis of the effectiveness of each computing agent. One of our evaluation metrics will be games statistics, such as ratio of wins to losses, time to win, and how many pieces lost.

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COSC2018LARSEN32693 COSC

Distinct Sound

Type: Undergraduate
Author(s): Nicholas Larsen Computer Science Harrison Cao Computer Science Minh Hoang Computer Science
Advisor(s): Liran Ma Computer Science

Distinct Sound
In the United States alone, 48 million people suffer from hearing loss. Sadly, about only 20 percent of them who could benefit from a hearing aid can afford to wear one. However, most people have a smartphone. Therefore, Distinct Sound strives to create an iPhone application that possesses similar functionalities of a conventional hearing aid at a fraction of the price. The two main objectives of our application are to remove background noise and to amplify sound in certain frequency ranges needed for speech comprehension. To complete those tasks, our app will take input sound, process that sound and amplify the frequencies that the user cannot hear as well, then replay the processed sound to the speakers through headphones. Those tasks will involve the fast fourier transform, and some sound processing to make sure that the sound does not have gaps. The app also provides a test to check the accuracy of the prescription in the current environment. If the current environment needs to adjust the prescription to make it more comfortable for the user, then a calibration test will work to fix the prescription according to the current environment. In conclusion, the research project will be considered a success if the application can successfully serve as a hearing aid with some functions that are unique on the market. It should benefit people who cannot afford traditional hearing aids.

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COSC2018REDDICK19284 COSC

An Ethereum-based Decentralized Voting Platform

Type: Undergraduate
Author(s): Luke Reddick Computer Science
Advisor(s): Liran Ma Computer Science

This paper covers a comprehensive implementation of a blockchain based voting platform. Blockchain, in its infancy, has shown remarkable use cases with cryptocurrencies and we would like to expand upon its possibilities. Voting is a system ripe with opportunity for blockchain; it requires security, consensus, and portability- all qualities inherited from blockchain technology. In this paper, we discuss the appeal of blockchain technology and why we want to elevate voting to 21st century technology. Next, we survey the needs of a voting platform and how blockchain might satiate those requirements. Finally, we propose a voting platform that will run on the Ethereum network and systematically discuss how this application could come to fruition.

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ENGR2018DEVOOGHT32056 ENGR

FDM 3D Printing Mechanical Properties

Type: Undergraduate
Author(s): Luke Devooght Engineering Melina Aguera Engineering
Advisor(s): Becky Bittle Engineering

In this experiment, the mechanical properties of 3D printed specimens of different printing parameters were tested under tension. The printing parameters of these specimens were: surface resolution, infill density, and print orientation. Parts were printed in Acrylonitrile Butadiene Styrene (ABS) plastic with a Fused Deposition Modeling (FDM) printer called the Stratasys UPrint SE Plus. Specimens were first printed similar to Stratasys published material properties standards and then tested to form a control on these known properties. Factorial sets of specimens using all various parameters were then printed and tested to create a reference table for future engineering projects.

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ENGR2018ELLIOTT14728 ENGR

Rare Earth Elements

Type: Undergraduate
Author(s): Lindsey Elliott Engineering
Advisor(s): Robert Bittle Engineering Efstathios Michaelides Engineering Ken Morgan Geological Sciences

From an engineering perspective, Rare Earth elements have the potential to transform technology in previously unprecedented ways. Their magnetic, luminescent, and electromechanical capabilities are allowing electronic devices to become more compact, reduce emissions, operate more efficiently, and cost less to produce and purchase. Such developments are proving beneficial to the economies of many developed nations because of their use in popular everyday consumer technologies as well as industries such as healthcare and education.

Along with this positive impact comes a political overlay that threatens the longevity of Rare Earth use. Presently, Rare Earths are expensive and dangerous to extract. This is largely due to the fact that they are not found together in large concentrations, so it is only economically feasible to extract them with another material, such as coal. The process of extraction is also hazardous and cumbersome; separating Rare Earths from other materials involves processes with high levels of emissions that may be dangerous to human beings if overexposure occurs. On the other hand, nations with more flexible safety and health regulations are investing in the development of Rare Earths and setting themselves apart as production leaders. Nations with more stringent health and safety regulations are becoming dependent on these nations to provide the Rare Earths for their applications. As a result, leaders in engineering industry can only benefit from Rare Earths if they develop systems that use Rare Earths more effectively than other materials commercially available and develop a reliable business relationship with a Rare Earth supplier. This condition is not likely to be encountered frequently in today's intricate social webs and economic systems.

The possibility of extracting Rare Earths through more efficient, safer processes is becoming recognized as a relevant topic of research. Additionally, investigation into alternatives to Rare Earths in some of the more common applications may allow for safer and less politically charged production methods for many 21st century advancements.

Through literary investigation, this research project seeks to highlight the main characteristics that makes Rare Earths desirable from an engineering perspective, proposed alternatives to Rare Earths based on engineering demands, and the direction of the Rare Earth industry as a result.

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ENGR2018KRAUSE24985 ENGR

Autonomous Vehicle with Room Mapping and Obstacle Avoidance

Type: Undergraduate
Author(s): Ben Krause Engineering Chris Prasai Engineering Bao Thach Engineering
Advisor(s): Morgan Kiani Engineering

The goal of this project is to design and construct a small modular autonomous car with room mapping and obstacle avoidance capabilities. The vehicle would be useful in cases where it is dangerous for a human to complete a task, or where it is more efficient to have an autonomous vehicle to scout ahead. A key design goal for this project was also to create an inexpensive platform for research into the realm of autonomous vehicles. The car uses lidar technology to create real time 2D room map and detect obstacles. It is programmed to explore rooms and move without human input. We designed the car with a powerful on board computer, enabling it to run complicated programs and operate without the need of an outside computer.

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ENGR2018TASMIM18442 ENGR

Development of a Device for controlling a Light Switch with a Remote Controller

Type: Undergraduate
Author(s): Seelay Tasmim Engineering Annie Le Engineering
Advisor(s): Sue Gong Engineering

The goal of this project is to develop a low cost and user-friendly device for remote actuation of light switches. We envision a product that is simple to install, easy to control via a remote, and able to function with a variety of light switch geometries. This device can minimize the inconvenience as well as the risk of injuries from turning the light on and off in the dark, especially for elderly people. For this target end user, the device must be simple and require no technical knowledge. Because of this, we have designed a mechanical actuator that will be mounted to the outside of a light switch without the need for tools and controlled by a simple button remote to be kept at the bedside.

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ENGR2018TOLBERT23197 ENGR

Tracking High Velocity Metal Objects

Type: Undergraduate
Author(s): Jacob Tolbert Engineering Lindsey Elliott Engineering Maya Hall Engineering John Hofmeister Engineering Darian Nezami Engineering Matt Spallas Engineering Cole Vallow Engineering
Advisor(s): Mike Harville Engineering Stephen Weis Engineering

Tracking and recording data from high velocity objects is a difficult task, especially when the object is hidden from view during portions of its flight path. When tasked with this problem, the process of solving it began with copious amounts of research into existing and developing technologies. From thermal imaging to radar detection, many options were explored.
Through a rigorous process of elimination to determine the most efficient and cost effective option, induction coils were chosen as the speed sensing device needed to track the desired objects. Normally when current is induced in one of these coils, there is an unchanging frequency of that current. However, when a conductive material passes through the center of a coil, the original frequency changes. This change can be monitored, giving valuable information about an object's location when evaluated over a specific time period.
After hours of bench top testing, several conclusions were made about the production and effectiveness of the induction coils. Chiefly, it was found that the smaller the induction coil diameter the more effective, the object passing through the coil has a larger effect if it does not pass through the exact center, and the "sweet spot" for the number of coil turns falls between 15-25 turns.

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ENGR2018VICTORIAN55314 ENGR

ACME senior design

Type: Undergraduate
Author(s): Brandon Victorian Engineering Matt Leonard Engineering Lauren Trost Engineering
Advisor(s): Robert Bittle Engineering

Senior design SRS submission:

For our presentation we hope to speak on three of our major groups of our senior design team:
Our first piece involves using programmable logic controllers (PLCs) that are used as the electrical interface between the programming and the mechanical system. Through its own ladder logic program, the code enables the PLC user to dictate when certain relays should be opened or closed for the purpose of turning on and off the vacuum supply and power sources. The PLC then collects data from the pressure transducers so that a signal indicating the next step is sent back to the design. After reading the pressure associated with a certain head, the user can then close a solenoid valve by sending a signal to it via the PLC which will stop the flow of air. With the PLC, the user is in control of where the flow is going to and is consequently, able to modify it through the code. Although the PLC is not a power supply, it does have the ability of processing information by receiving and sending out specified actions, set by the user, to different electronic and mechanical components.
The second piece is based of a tool from a company called pave more. The “pave more” design is a design that picks up bricks from the hack to a separate location to pack them. The design uses separate heads that pick-up bricks using foam that creates a seal on the brick. The heads are connected to a vacuum that allows us to pick up the bricks efficiently. The heads are each on their own spring system that allows them to be picked up at different heights. They are also each on a separate solenoid valve that will sense a missing brick and close the valve to still allow the system to pick up the bricks. The vacuum system is connected to a filter to protect it from the dust and dirt that are on the bricks.

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ENSC2018CLARK52950 ENSC

The Impact of Residential Swimming Pools on Bat Populations

Type: Undergraduate
Author(s): Delaney Clark Environmental Sciences
Advisor(s): Tamie Morgan Environmental Sciences Victoria Bennett Biology

Habitat loss due to urbanization is a primary cause of declining bat populations globally. As a result of this, research has been conducted to review swimming pools as an alternative source of water for bats in urban areas. After collecting data, GIS analysis utilizing color infrared imagery was performed to assess the impact that residential swimming pools have on bat populations.

(Presentation is private)

ENSC2018GILLIAM49891 ENSC

External Influences Impacting the Fluctuations in Texas Groundwater

Type: Undergraduate
Author(s): Dorothy Gilliam Environmental Sciences
Advisor(s): Tamie Morgan Environmental Sciences Becky Johnson Environmental Sciences

In the state of Texas, groundwater resources are utilized for irrigation, mining, municipal use, manufacturing, livestock and steam electric. Over the past 20 years however, there have been shifts and significant trends in groundwater pumpage that can be attributed to changes in annual precipitation, drought, declining industries, and the status of livestock. A multi-year GIS analysis was conducted to analyze trends in Texas Groundwater and the overall factors that impacted pumpage.

(Presentation is private)

ENSC2018INGLIS50949 ENSC

Texas Rare Plants

Type: Undergraduate
Author(s): Emily Inglis Environmental Sciences
Advisor(s): Tammie Morgan Geological Sciences

This project will map 2,000-4,000 rare plant species found in Texas. Most of these species have geocoordinates, with some only down to the county-level. These georeferenced plants will be overlaid on to soil type, precipitation, and land development, topography, and ecosystem type maps. This analysis will explain why these habitats are ideal for the rare plants in Texas. Understanding the habitats of these rare plants is important in preserving endangered botanical species. This could lead to a better understanding of this rare biota.

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ENSC2018LAM39506 ENSC

Surface Property of Organic Sorbent Derived from Coffee Grounds

Type: Undergraduate
Author(s): Amy Lam Environmental Sciences
Advisor(s): Omar Harvey Environmental Sciences

On average, Americans generate about 11.4 million kilograms of spent coffee grounds per day. That is an equivalent weight of a thousand full-size school buses, every day. Most of this coffee is discard, where it eventually ends up in a landfill. However, if recycled or reused this commonly discarded material has many potential uses including as a pest repellent or garden fertilizer. Another use is as a sorbent to remove water contaminants. This means that coffee grounds have the potential to be used as a key component in carbon-based water filters. Evidence from recent research conducted in our laboratory at Texas Christian University shows that charred coffee grounds can effectively remove lead contamination from water. My research will further this work by identifying 1) the specific properties of charred coffee grounds that allows for the removal of lead from water and 2) the optimal temperature for producing charred coffee grounds for water filtration. With the use of infrared spectroscopy and other materials characterization techniques, I will study the properties of charred coffee grounds produced from regular Folgers coffee and an Ethiopian-blend at 250 ℃, 350 ℃, and 450 ℃.

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ENSC2018LAURENTI30255 ENSC

A Space-Time Analysis of Multi-Year Air Quality Data in Fort Worth and Houston in order to Quantify Cancer Risk

Type: Undergraduate
Author(s): Alec Laurenti Environmental Sciences
Advisor(s): Omar Harvey Environmental Sciences Tamie Morgan Geological Sciences

BTEX compounds (benzene, toluene, ethylbenzene, and xylene), and specifically benzene, have been linked to cancer in humans. This project will allow me to develop a map to quantify risk of cancer based the amount of BTEX compounds that have been determined to be in the air. Air pollutant data was gathered by TCEQ using automated gas chromatographs. I collected this data for different monitoring stations in the DFW area in order to compare the differences with Houston. This data was then used to create a map in ArcGIS in order to visualize higher pollution areas. The contaminant levels will then be used with the recommended health exposure levels in order to create a map of risk corridors. This is useful information as it allows individuals to be aware of their personal exposure to these compounds based on the time spent in an area.

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ENSC2018PAYBLAS44391 ENSC

Evolution of Groundwater Quality and Source Tracking of Nitrate Contamination in the Seymour Aquifer, Texas

Type: Undergraduate
Author(s): Caitlin Payblas Environmental Sciences
Advisor(s): Omar Harvey Environmental Sciences

Nitrate contamination of groundwater in the Seymour Aquifer is a well-known issue that has been documented since the 1960's. Concentrations as high as 35 ppm NO3-N have been reported, which is a startling 3.5 times the EPA allowable standard for drinking water. While most water from the Seymour Aquifer is used for agricultural irrigation, a portion is still used for domestic purposes and therefore poses a risk to human health. While this problem may have been recognized, the specific source of this contamination remains unknown. Three potential sources of nitrate within the aquifer are being considered in this study—the geological makeup of the aquifer, the agricultural contribution of nitrate from fertilizers, and the historical land use change of the area above the aquifer. My research will combine various analytical and geospatial technologies in order to 1) assess the evolution of groundwater in the Seymour Aquifer since the 1960's, and 2) to determine the source of the high concentrations of nitrate in domestic wells situated on the aquifer. Readily available groundwater quality data from the Texas Water Development Board will be used in conjunction with geospatial analysis and chemical analysis to identify changes in the aquifer's water quality over time. Nitrogen and Oxygen stable isotopic analysis will be used to determine the source of the contaminant. After a thorough analysis of the site area via the aforementioned methods and technologies, a thorough portrait that depicts the source of nitrate contamination in Texas's Seymour Aquifer ought to be painted.

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ENSC2018PUETT4458 ENSC

If it weren’t for the neighbors! – urban habitats can benefit bats.

Type: Undergraduate
Author(s): Richard Puett Environmental Sciences Ellen Hall Environmental Sciences
Advisor(s): Tory Bennett Environmental Sciences

Bats are critical to their surrounding environment, providing numerous beneficial ecosystem services. For instance, they are natural pest controllers, and in urban environments they can control the mosquitoes that cause West Nile Virus. Nevertheless, loss and degradation of habitat, along with disease, have led to declining bat numbers. Restoring and creating suitable habitat will certainly help encourage bats, but first we need to know what resources bats need to survive, such as water. Many available water resources in urban areas, such as streams, ponds, and drainage ditches are ephemeral and dry up during the hot Texas summers. We believe that bats are able to utilize swimming pools in Texas urban areas, thus we explored this by radio-tracking bats in a local park, Foster Park in Fort Worth. We caught bats in this park using a technique called mist netting. Upon capture, we attached a radio-transmitter which emits a signal that can be picked up by a hand-held receiver. We then followed the bats using the transmitter’s signal and triangulated their position every minute to map their nightly routine. From March to September 2017, we tracked a total of 10 evening bats (Nycticeius humeralis). Using ArcGIS, we mapped the bats flight paths and determined home range sizes. From March to May, and September, we found that bats tracked tended to restrict their movement and remained within or near to the park, however from June to August the bats expanded their home ranges and moving longer distances into local neighborhood. This expansion coincided with drying up of water sources within the park, and included areas with swimming pools. Our finding supports the hypothesis that urban habitats have the potential to maintain healthy bat populations, which in turn can aid bat conservation.

(Presentation is private)

ENSC2018WILSON22698 ENSC

Potential Distributed Power Generation for U.S. Border Stations in Texas and New Mexico

Type: Undergraduate
Author(s): Clare Wilson Geological Sciences
Advisor(s): Becky Johnson Environmental Sciences

Intermittent power outages at Texas and New Mexico border stations has caused significant delays in customs services and information losses through computer shutdowns. The U.S. General Services Administration approached us to address these power quality problems at the border stations through a review of potential distributed generation sources through microgrids to “combat or support” these frequent power outages. The overall aim aside from solving power outages and brown outs at stations is potentially addressing the implementation of renewable energy sources as a power generation for microgrids and coming closer in compliance with Executive Order 13693, “Planning for Federal Sustainability in the Next Decade”. Our approach includes analyzing background information through analysis of GSA documentation and current studies on implementing microgrids in a variety of locations. Current data suggests proposing wind power, solar power, and battery storage based on size and locations of border stations. However, results are pending data collection and GSA input.

(Presentation is private)

GEOL2018BURNS41328 GEOL

Geomechanical Relationship Between the Maness Shale and Lower Eagle Ford, San Marcos Arch, Texas

Type: Undergraduate
Author(s): Alec Burns Geological Sciences
Advisor(s): Richard Denne Geological Sciences

The geomechanical properties of Eagle Ford cores from the San Marcos Arch were measured by hardness tools to test that calcareous rocks are stiffer than the clay-rich shales. Results were quantified and graphed to reinforce the idea that the Maness shale could be more ductile than the superimposing Eagle Ford Shale.

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GEOL2018BURNS56887 GEOL

Facies modeling of the Lower Williams Fork, Piceance Basin, CO

Type: Undergraduate
Author(s): Alec Burns Geological Sciences
Advisor(s): Xiangyang Xie Geological Sciences Tamie Morgan Geological Sciences

Lithology and facies of the Lower Williams Fork in the Piceance Basin, Colorado were interpreted using spectral gamma ray logs on Petrel. Models were created using this correlated data to predict the facies in an area with no well data. Different modeling methods will be used, such as object modeling and sequence indication simulation to compare and search for the best fit. Published outcrop measurements were used to constrain subsurface geobody geometry. Models were also used to estimate reservoir rock potential in the Lower Williams Fork.

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GEOL2018OHRMUNDT26503 GEOL

Andesitic pyroclastic intrusions injected laterally into weak lacustrine sediments within a Mesoproterozoic volcanic arc succession, Barby Formation, SW Namibia

Type: Undergraduate
Author(s): Sierra Ohrmundt Geological Sciences
Advisor(s): Richard Hanson Geological Sciences

The 1.2 Ga volcanic arc rocks in the Barby Formation are well exposed in desert terrain in SW Namibia - this formation records the establishment of a major continental margin arc following earlier accretionary events. Recent field work has shown that large portions of the formation consist of pyroclastic fall deposits erupted from small volcanoes (fissures and scoria or spatter cones) in a region with poor drainage and abundant lakes.

Detailed mapping of a well-exposed section of the Barby Formation provides a cross-sectional view of a succession of pyroclastic fall units intercalated with planar bedded lacustrine sediments. Massively bedded units up to ~80 m thick show abundant bombs up to 60 cm across in a matrix of fluidal to angular lapilli, indicating deposition close to source vents undergoing primarily Strombolian-type eruptions. Hypabyssal dikes and sills are common, often cutting through the massively bedded pyroclastic units.

Also present are pyroclastic deposits that intrude lacustrine sedimentary packages at 12 locations spread out over a horizontal distance of ~600 m and a vertical stratigraphic sequence of ~300 m. These deposits contain similar bombs and lapilli as the pyroclastic fall deposits, but show clear fluidal intrusive relations with adjacent sedimentary units. In most cases, zones of peperite are formed in between the pyroclastic intrusions and the lacustrine sediments, consisting of fluidal bodies of vesicular basaltic andesite mingled with fine-grained sediment with preserved lamination. We infer that jets of intrusive pyroclastic material were blasted laterally into weak, unlithified lake sediments from one or more vent conduits feeding explosive eruptions at the surface; these jets are likely to have been forced out by collapse of the conduit inward. Fluidization of the sediment would have occurred as pore water was converted to steam, which would have facilitated lateral motion of the pyroclastic jets.

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GEOL2018OHRMUNDT44993 GEOL

Mapping geology of SW Namibia using Landsat-8 band ratios

Type: Undergraduate
Author(s): Sierra Ohrmundt Geological Sciences
Advisor(s): Tamie Morgan Geological Sciences

Landsat-8 data was used to test the effectiveness of using spectral analysis and remote sensing in the differentiation of lithological units and mapping geology in Namibia. The study area is located in SW Namibia, in an arid region with little vegetation, making it an ideal place for remote sensing analysis. Different color composites and band ratios were compared to find the image providing the most geologic information and highest contrast between units. A false color composite (6,3,2 in red-green-blue) was first created to to show differences in bare earth, and from there, various band ratio combinations were created. Geologic maps were used to verify the results and select the best band combination. The best color composite image was created using band ratios from (7/6, 6/5, 4/2), and allowed identification of lithological units and vegetation. The results show that it is possible to draw valid lithological conclusions from spectral patterns, and that high quality imagery can be used to update existing geologic maps or used for exploration.

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GEOL2018PATE42788 GEOL

Drainage Area Climate Classification

Type: Undergraduate
Author(s): Jacob Pate Geological Sciences
Advisor(s): Tamie Morgan Geological Sciences

SRS Abstract Drainage Area Climate Classification

For my SRS project I will be determining the climate(s) within a given polygon. The Polygon size and shape will be determined from a specified drainage area for a given stream. I will be using over 400 stream data points with a series of drainage area shape files given to me by Nicole Wilson. I will base the climate on the gauge site location within the drainage area. The koppen climate classification scheme will then be used to specify each drainage area.

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GEOL2018WALKER38503 GEOL

Cobalt Cache: Finding a Solution for Runaway Cobalt Demand

Type: Undergraduate
Author(s): John David Walker IV Geological Sciences
Advisor(s): Tamie Morgan Geological Sciences

Public awareness of human rights violations in cobalt-rich mines of the Democratic Republic of Congo have American cobalt consumers scrambling for reliable suppliers to meet rising demand, with uncertainties about futures in cobalt's supply chain. Global cobalt production supply forecast falls short of global demand forecast for the next ten years, even with the completions of major planned mined projects. The exponential increase in demand for cobalt results from its utility in personal electronics batteries, electric vehicle batteries, and jet engine construction. Chemical extracting operations whose sole purposes are to extract cobalt are not profitable in the status quo, leaving a window of opportunity for reclaimed cobalt to take hold in the market. This project studies consumer patterns to determine "urban mine" qualities in Texas. The resulting analysis exists to be cross-applied to other states to determine target regions best suited for cobalt reclamation strategies in hopes of securing America's geopolitical mineral stability.

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INTR2018FRANKLIN55390 INTR

Extinction Learning Deficits Precede Acquisition Deficits in a Mouse Model of Alzheimer’s Disease

Type: Undergraduate
Author(s): Christian Franklin Biology Julia Peterman Psychology Jordon White Psychology
Advisor(s): Michael Chumley Biology Gary Boehm Psychology Meredith Curtis Biology

Alzheimer’s disease (AD) is a progressive brain disorder and the most common form of dementia. The disease gradually destroys brain cells, leading to confusion, erratic behavior, and severe loss of memory. Alzheimer’s is eventually fatal, and no treatment or cure has been discovered. Researchers aim to better understand Alzheimer’s pathology through the use of a transgenic mouse model of AD, the 5xFAD mice. A previous study by Bonardi et al. (2011) has shown that another model of AD, the APP/PS1 mouse, exhibits a deficit in extinction learning before it displays a deficit in acquisition. We aim to determine if this same trend exists in 5xFAD mice, despite having more extensive genetic mutations. Learning will be assessed using the Contextual Fear Conditioning (CFC) paradigm, where the mice are introduced to an environmental context and experience a mild aversive stimulus. When reintroduced to the context 24 hours later, mice will freeze if they acquired a memory for the pairing of an aversive stimulus with the novel context. Freezing is an instinctive rodent fear response. After repeated trials of exposure to the environment in the absence of an aversive stimulus, the mice gradually freeze less. This is indicative of new learning of the environment no longer being paired with the stimulus, or extinction of the initial association. The 5xFAD mice typically exhibit impaired acquisition by 6 months of age as compared to wild type mice. The present study examined if the 5xFAD mice would display a deficit in extinction learning prior to this deficit in acquisition. Preliminary data indicate that 5xFAD mice, like APP/PS1 mice, show a deficit in memory extinction before they exhibit a loss of memory acquisition. Three-month old FAD mice extinguish more slowly than three-month old wild type mice, but show no difference in acquisition. This research is important because it indicates alternative cognitive measures may allow for earlier diagnosis of neurodegenerative diseases, such as AD.

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INTR2018KINSINGER59613 INTR

Reducing ROS and Chelating Metal Ions in Neuronal Cells Using Novel Compounds

Type: Undergraduate
Author(s): Olivia Kinsinger Interdisciplinary
Advisor(s): Giridhar Akkaraju Biology

It is estimated that 45% of people over the age of 85 in the U.S. suffer from Alzheimer’s disease. Patients with Alzheimer’s disease, which is characterized by cognitive deficiencies and memory loss, have higher concentrations of amyloid plaques in brain tissue than patients without the disease. Abnormal levels of transition metal ions Fe, Zn, and Cu in brain tissue are associated with amyloid beta plaques and also have been shown to catalyze the generation of excess reactive oxygen species (ROS) and cause oxidative stress. The combination of the ROS generation and the amyloid plaque formation results in neurodegeneration, which ultimately causes the memory loss and ultimate death associated with Alzheimer’s. We have synthesized the compounds L2 and L4 which are designed to be chelating agents of metal ions and also scavengers of ROS. We hypothesize that due to their chelating properties and pyridol groups, L2 and L4 should reduce oxidative damage in neuronal cells by chelating metal ions and scavenging radicals. Furthermore, we hypothesize that due to its extra pyridol group, L4 will be a stronger antioxidant than L2. The cytotoxicity of the compounds was tested on HT-22 neuronal cells. Neuronal cells will be treated with BSO, a compound that induces formation of ROS, in the presence and absence of L2 and L4. If our hypothesis is correct, our compounds should reduce the oxidative damage induced by BSO, and L4 should be more effective at doing so than L2.

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